An Evaluation of the Effectiveness of Eukaryotic Dna Extraction from Burial Soil Samples
نویسنده
چکیده
DNA is a valuable resource as a unique identifier of an individual’s dietary habits, sex, ancestry, and a variety of other characteristics. Due to a variety of factors that contribute to genetic decay, forensic and bioarchaeological investigators have limited outlets in which to extract viable DNA after most of the organic materials have fully decomposed. This preliminary research focused on extracting DNA from the soil surrounding buried Sus scrofa domesticus cadavers to confirm the presence of viable and analyzable DNA. After a decomposition period of five months in Montana, soils were collected at incremental distances above the remains and sequenced to identify endogenous DNA. The primers targeted a genetic template specific to Sus scrofa, and sequence fragments were confirmed by sequence submission to BLAST. The results of this study indicate basic temporal characteristics and leaching attributes of DNA preservation in soils surrounding burial sites with an interred cadaver. The conclusions of this study promote the collection of soil in crime scene investigation procedures, or catalyze research opportunities across biological and archaeological disciplines.
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تاریخ انتشار 2016